含挥发油1.8%,油中主成分为右旋薄荷酮(α—Menthone)、消旋薄荷酮、少量右旋柠檬烯(α—Limone-ne)。地上部分含挥发油1.3%,穗含挥发油约4.11%,油中主成分为d-薄荷酮(d-menthone)约42.9%,消旋薄荷酮、l-胡薄荷酮(l-pulegone)约33.9%及考试,大收集整理少量d-苧烯(d-limonene),另尚含γ-蒎烯。此外,荆芥穗中尚含荆芥甙A、B、C(schizonepetosideA,B,C),(1S,4E)-9-O-β-D-glucopyranosyl-pmenth-4(8)-en-3-one,apigenin-7-O-β-D-glucoside,luteolin-7-O-β-D-glucoside和橙皮甙(hesperidin)。