4ԼɫӦ
չ⼰£ֽͨ߷Ӧ۲üԼɫ仯ڼͪһ塣Уðֵɫ仯ÿ漴ȥ̼ˮҺֵɫÿȴɫ
⣬üԼķӦɰijЩṹ磺
ٶͪڼҺпתӦ칹塪ͪ,Գȡɫ
ͪͲͪᡢµĽṹ
ڻͪڼҺȳʻɫͨΪɫݴ˿ͪ
ۻͪﵱڶǻȡ3, 4-ǻȡʱ,ڼҺвȶױɻɫɫɫ
ȡ
1. ͪһȡȡԭṹ֮Ĺϵ
1ܼȡ ûͪʼԲͬѡòܼͬȡɴﵽƴĿġ
2ȡ ͪһԣˮȴˮڼˮʿüˮȡٽˮȡҺԣͪ༴ɳ˷У«ƤաȡӦ
üᷨȡʱӦעüҺŨȲ˹ߣǿ£ʱƻͪĸˡڼữʱҲ˹ǿɪΣʹĻͪܽ⣬ͲƷʡ
3̿
Ҫľƹֲͨļ״ȡУִμ̿裬ãֱԼҺͪӦʱΪֹ˹ռ̿ĩ÷ˮм״7%/ˮ15%/Һϴѡ
2. ͪҪ뷽
1ɫ ͪﳣõй轺άط۵ȡ⣬Ҳþȡ
轺ɫ ˷Ӧ÷Χ㣬Ҫڷͪͪͪ߶ȼѻĻͪͪࡣ
ɫ Էͪ˵ǽΪǿҪȡڻͪǻĿλüܼ֮ͪγĴСͪӾϴʱɣ
ԪͬϴȺ˳һա˫աաԪ
ĸǻϴٶȼӦǻĿͬʱǻλöҲӰ죬Դʻλλǻλǻϴ˳Ϊλǻͪжλλǻͪ
ͬͻͪȺ˳һͪͪͪͪ
зˡ˫ױʲͪӦĶͪϴѡ
ϾSephadex gelɫ ڻͪķ룬ҪͺŵSephadex-GͼSephadex-LH20͡
ϾͪĻƣ
ͪʱҪáԻ̶ͪȡǻĿͪʱɸáϴʱͪǰɴС˳壬
2ݶpHȡ
ݶpHȡʺǿͬĻͪԪķ롣ݻͪԪǻĿλòͬǿҲͬʣԽлܼѣ5%NaHCO35%Na2CO30.2%NaOH4%NaOHҺȡﵽĿġһɴ£
ԣ 7, 4- OH 7-4-OH һOH 5-OH
NaHCO3 Na2CO3 ڲͬŨȵNaOH
3ݷijЩضŽз
ڻͪɷֵĻУڶǻɷ˽ṹijɷ֣Ǧη롣ڶǻijɷֿɱǦڶǻijɷֿɱʽǦݴ˿ɽɷַ롣ͪɷֻʵķȻճҺлᡢʡȣڶǻʵȣʱҲɱǦﵽȥʵĿġͪǦɵij˼ҴУͨH2Sиֽ⣬˳ǦҺпɵõͪ
ġͪҩʵ
1. ΪȽⶾҩҪЧɷֻվпãΪɫ뾧ˮڼ״Ҵʱױˮʡ
2. Ҫܻͪͪӹ״Ѫļá
3. ҶĻͪлͪͪա˫ͪͶصȡŹ״ѪܺѪá
塢ṹⶨ
1. ͪɫ
1ֽɫףPCڷȻͪPĻԻͪ˵һһչijִܼҪǸݷԭз롣ڶչܼúˮҺҪǸԭз롣
2轺ɫ ڷԻͪϺá
3ɫ ÷ΧϹ㣬رʺڷ뺬ǻĻͪࡣھԻͪǿչҪǿļԡڴչкдˮ